Sartorius #2 eBook - 2019 - 21

Importance of mAb Discovery and Development in Immunotherapy

our company can be used with any cell line, any production process, and any media.
This flexibility of our system means that it can be used in a wide variety of experiments needed during the development of antibodies."
Another issue is the post-translational modification of antibodies. These modifications
can vary between cell types used to make the antibodies. Dr. Brady adds that because
cells that will be used in manufacturing are easy to employ with the company's
platform, differences in post-translational modification introduced by the use of
different cell lines is eliminated. Thus, says Dr. Brady, "data generated on our platform
are more relevant."
Automation Comes to Ab Cloning
At the conference, Kurt Klimpel, Ph.D., field application specialist, product development at SGI-DNA, talked about synthetic genomics. He said that his company can
automate cloning using a DNA genomics workstation, BioXp™ 3200, which permits
hands-free, rapid, antibody library construction.
He cited as a case study the antibody engineering group at VIB in Belgium. The group
designed and assembled a family of nanobody sequences using the BioXp instead of
a traditional manual workflow. The goal of the project was to clone 30 different DNA
constructs into a VIB proprietary expression vector.
"The conventional method for doing this involves laboriously designing and ordering
primers to build the fragments for cloning into the first vector; transforming this
into bacteria, plating, picking clones, using PCR amplify for verification, and finally
sequencing for confirmation-and this is only the first half of the project. If all goes
well, this takes at least a month of dedicated time for a molecular biologist," explains
Dr. Klimpel.
The project involves submitting the desired DNA sequences. From there, SGI-DNA
designs the oligonucleotides and other reagents, sending these reagents on to the
customer. The customer then loads the reagents into their BioXpand and runs a
customized overnight process that both creates the doubled-stranded DNA (dsDNA)
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Sartorius #2 eBook - 2019

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