Agilent eBook - 15

Best Practices with an Agilent Parallel CE System

A 9,000
UM

8,000
7,000
6,000
RFU

LM

5,000
4,000
3,000
2,000

Size (bp)

800
1,000
1,500

500
600

400

300

250

200

150

100

0

50

1,000
1

The HS Small Fragment kit has a DNA sizing
range of 50 to 1,500 bp. A DNA smear was
separated on the 5200 Fragment Analyzer
system with the HS Small Fragment kit over
a concentration range of 5,300 to 78 pg/µL
(Figure 3A). The smear size remained
consistent at 295 bp, with no significant
change over the entire concentration range
of the kit (Figure 3B). Both the Small Fragment
kits and NGS Fragment kits provide consistent
fragment and smear sizing across the
concentration range of the respective kits.

B
Smear size over concentration range
Concentration (pg/µL)

5,300

2,900

1,350

650

320

160

78

Average (bp)

295

294

294

294

296

296

295

Standard deviation

3.5

2.6

3.2

4.3

6.2

5.9

9.4

1.2 %

0.9 %

1.1 %

1.5 %

2.1 %

2.0 %

3.2 %

Precision % CV

15

| GENengnews.com

Figure 3. DNA smear separated by the 5200
Fragment Analyzer system with the HS Small
Fragment kit over a concentration range of
5,300 to 78 pg/µL. (A) Electropherogram
overlay. (B) Table of average smear size,
standard deviation, and precision (% CV). DNA
smear sizing remained consistent over the
entire concentration range. n = 10; LM = lower
marker; UM = upper marker.



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Agilent eBook

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