Assay and Drug Development Technologies - 9

ADVANCES IN HIGH CONTENT SCREENING
users to be proficient in image analysis to create their own
scripts to measure different cellular parameters. Recently, an
additional class of BioApplications has been introduced to
expand the flexibility of these turnkey software modules.
''General'' BioApplications provide a high degree of flexibility
by including a large set of cellular domain measurements.
These measurements, along with an expansive list of assay
control parameters, enable a broad biological range of applicability.
Users can customize the General BioApplication
protocols for their most interesting biological target(s).
FIG. 2. HCS data visualization capabilities. A multipanel data visualization application for where large HCS datasets can be interactively
viewed, validated, and analyzed. In the field-level image panels, images of cell populations are displayed one field at a time. Here, data
from a four-channel assay are displayed along with the color overlays that delineate objects and the cellular subregions used for
algorithmic data extraction. In the cell-level image panels, four-channel images of a single cell selected by the user from the field-level
images are shown. Here, the multiparameter image of a single cell chosen from a field-level image is shown with an overlay marking the
regions of interest. The numerical data extracted from all the cells in the well are displayed in the single cell feature data panel. Here, 140
columns of data extracted from the images displayed in the single cell image panels are highlighted. Users can select cell population data
to be shown in the well-level data display panel. Choices include line plots, histograms, and scatter plots.
ª 2022 MARY ANN LIEBERT, INC. ASSAY and Drug Development Technologies 9

Assay and Drug Development Technologies

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