IsoPlexis eBook - 11

I S O P L E X I S ' U N I Q U E , P R E D I C T I V E B I O LO G Y
Furthermore, CD8+ hYP7 CAR-T cells had a PSI 8 to
11 times higher, thus more polyfunctional, than the
CD4+ hYP7 CAR-T cells co-cultured with Hep3B and
G1 cells. The researchers also discovered that HCC

contribute to the discrepancy between the efficacies
of the CAR constructs.2

Conclusion

patient-derived polyfunctional CD8+ hYP7 CAR-T

IsoPlexis' single-cell functional phenotyping has

cells contained effector cytokines (granzyme B, IFN-γ,

revealed critical functional drivers that correlated

and perforin) as well as cytokines not secreted by

with CAR-T cell persistence in both BL-ALL and HCC

CD8+ hYP7 CAR-T cells from healthy donors (TNF-α,

indications. This demonstrates how early information

MIP-1β, and IL-8).

on T cell polyfunctional capacity provides clear value

Sixty-six percent of mice treated with the hYP7

to facilitate decisions on lead candidates in the

CAR-T cells had their tumors eliminated by week 3,

development of cell therapies.

while mice treated with the HN3 CAR-T cells showed

References

no reduction in tumor burden. The hYP7 CAR-T
treated mice also continued to be free of tumors after
additional Hep3B cells were introduced. The authors
hypothesized that differences in persistence, integration site preferences, and T cell polyfunctionality may

1	 Fousek K, Watanabe J, Joseph S, et al. CAR T-cells that target acute
B-lineage leukemia irrespective of CD19 expression. Leukemia (2020).
doi.org/10.1038/s41375-020-0792-2
2	

Li D, Li N, Zhang YF, et al. Persistent Polyfunctional Chimeric Antigen
Receptor T Cells That Target Glypican 3 Eliminate Orthotopic
Hepatocellular Carcinomas in Mice. Gastroenterology 2020 Feb 11. pii:
S0016-5085(20)30211-0. doi.org/10.1053/j.gastro.2020.02.011.

Improve Immune Persistence in Solid Tumor
with Unique Single-Cell Proteomics Solutions
IsoPlexis' functional phenotyping exhibits unique utility for
understanding persistence and overcoming tumor-immune
challenges in cancer immunology. Single-cell proteomics has
identified polyfunctional cells correlating to increased proliferation, homing, and persistence of anti-tumor T cells in vivo.

Learn more in this App Note

GENengnews.com

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http://www.doi.org/10.1038/s41375-020-0792-2 http://www.doi.org/10.1053/j.gastro.2020.02.011 https://www.isoplexis.com/literature/isoplexis-proteomic-product-suite-for-cancer-immunology/ https://www.isoplexis.com/literature/isoplexis-proteomic-product-suite-for-cancer-immunology/ http://www.GENengnews.com

IsoPlexis eBook

Table of Contents for the Digital Edition of IsoPlexis eBook

Contents
IsoPlexis eBook - 1
IsoPlexis eBook - 2
IsoPlexis eBook - Contents
IsoPlexis eBook - 4
IsoPlexis eBook - 5
IsoPlexis eBook - 6
IsoPlexis eBook - 7
IsoPlexis eBook - 8
IsoPlexis eBook - 9
IsoPlexis eBook - 10
IsoPlexis eBook - 11
IsoPlexis eBook - 12
IsoPlexis eBook - 13
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IsoPlexis eBook - 15
IsoPlexis eBook - 16
IsoPlexis eBook - 17
IsoPlexis eBook - 18
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