Foot & Ankle International - 2017 FAI Supplement - 42S
Foot & Ankle International, 38(S1)
DOI: 10.1177/1071100717S00038
©The Author(s) 2017
Clinical Application of Immunoassay for
Identification of Staphylococcus aureus
and Monitoring Treatment Response in
Diabetic Foot Infection
Irvin Oh, MD, Benjamin Smith, BS, Alex
Grier, MSc, Steven Gill, PhD, John Daiss, PhD
Category: Basic Sciences/Biologics, Diabetes
Keywords: Diabetic foot infection, Diabetic foot ulcer,
Immunoassay
Introduction/Purpose: Currently, there is no sensitive and
specific diagnostic tool for identifying the active pathogen
in a polymicrobial environment such as diabetic foot infection (DFI). In addition, monitoring the success of antibiotic
42S
treatment is limited to clinical signs and nonspecific inflammatory markers. Consequently, surgeons are often forced to
make interventional decisions without adequate prognostic
information. While DFI are generally polymicrobial, about
50% prominently include Staphylococcus aureus (SA). We
investigated applicability of utilizing a novel diagnostic
immunoassay that measures the patient's current production
of anti-SA antibodies (IgG) to accurately diagnose SA and
monitor its pathogenic activity. We hypothesize that 1)
compared to standard culture, the immunoassay has a higher
sensitivity to detect SA and 2) able monitor changes in
pathogenic activity of SA in DFI.
Methods: From July 2015 to August 2016, we enrolled 20
diabetic patients with DFU who displayed clinical symptoms and signs of infection which necessitated hospitalization and undertook initial foot salvage therapy (FST):
irrigation and debridement followed by wet-to-dry dressings and 6 weeks of intravenous antibiotic treatment. At
weeks 0, 4, 8 and 12, the infected DFUs samples were
obtained for standard culture and 16S rRNA microbiome
analysis. Whole blood and serum samples were collected to
measure the abundance of anti-SA IgG in the serum and in
Foot & Ankle International 38(1S)
Table of Contents for the Digital Edition of Foot & Ankle International - 2017 FAI Supplement
TOC 1
TOC 2
TOC 3
TOC Page 4 + Verso
Editorial Board
President's Introduction
AOFAS Annual Meeting Abstracts 2017
AOFAS Annual Meeting Abstracts 2017
Foot & Ankle International - 2017 FAI Supplement - CT1
Foot & Ankle International - 2017 FAI Supplement - CT2
Foot & Ankle International - 2017 FAI Supplement - Cover1
Foot & Ankle International - 2017 FAI Supplement - Cover2
Foot & Ankle International - 2017 FAI Supplement - i
Foot & Ankle International - 2017 FAI Supplement - TOC 1
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Foot & Ankle International - 2017 FAI Supplement - TOC 2
Foot & Ankle International - 2017 FAI Supplement - 1A
Foot & Ankle International - 2017 FAI Supplement - 1B
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Foot & Ankle International - 2017 FAI Supplement - TOC 3
Foot & Ankle International - 2017 FAI Supplement - vii
Foot & Ankle International - 2017 FAI Supplement - TOC Page 4 + Verso
Foot & Ankle International - 2017 FAI Supplement - Editorial Board
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Foot & Ankle International - 2017 FAI Supplement - President's Introduction
Foot & Ankle International - 2017 FAI Supplement - AOFAS Annual Meeting Abstracts 2017
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Foot & Ankle International - 2017 FAI Supplement - AOFAS Annual Meeting Abstracts 2017
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