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Otolaryngology-Head and Neck Surgery 163(1S)
Presentation schedule is subject to change. The Official Program Abstracts supplement does not reflect changes made after August 10, 2020.
establish a control. The positioning of the CHD was then
tested using a surgical retractor with a flexible arm. The tip of
the CHD was directed to reach the round window. The EA was
mounted onto the CHD. An infusion pump was then connected to the CHD and set to drive it at a predetermined insertion velocity of 0.4 mm/s. A postinsertion computed
tomography (CT) scan was performed for radiographic evaluation of the insertion.
Results: The CHD can (1) be positioned suitably, as it
attaches to the human head, stands alone, and achieves appropriate direction for EA insertion; (2) respond to the hydraulic
actuation once placed in the desired position; and (3) introduce the EA into the human cadaver cochlea. Postinsertion CT
demonstrates intracochlear positioning of the EA.
Conclusions: The first attempt to perform a CI EA insertion
using the CHD was successful. Further testing to validate its
performance and reliability is ongoing.

Otology/Neurotology

Identifying Eustachian Tube and Middle
Ear Abnormalities in the Mechanically
Ventilated Patient
Eduardo Lopez Orozco, MD;
Noraima Leticia Rodriguez Guevara, MD
Objectives: Up to 2.8% of hospitalized patients receive
mechanical ventilation in the United States. Patients under
mechanical ventilation develop a series of complications
related to sedation, supine position, and positive-pressure ventilation, such as pressure ulcers, pulmonary embolism, and
ventilation-associated pneumonia. Although often overlooked, Eustachian tube and middle ear abnormalities are very
common in the mechanically ventilated patient. In these
patients, factors such as supine position, mucociliary hypofunction, lack of swallowing, and devices that physically
obstruct the Eustachian tube pose a challenge to the adequate
functioning of the middle ear.
Methods: A transverse observational study was performed in
which otoscopy, tympanometry, and nasopharyngeal endoscopy were performed in intubated patients with varying duration of orotracheal intubation. The study was performed at
Hospital Civil de Guadalajara Fray Antonio Alcalde in Mexico.
Results: Fifty patients were included, with ages ranging from
18 to 78 years. The most common diagnosis was traumatic
brain injury. Days under mechanical ventilation ranged from 2
to 14. In total, 100% of patients had alterations in their tympanometry results, with the Jerger type B curve being the most
prevalent of those. We found nasopharyngeal pooling of secretions and mucosal edema during the endoscopy of all patients.
Conclusions: In this study, we found that every patient under
mechanical ventilation had some kind of middle ear or
Eustachian tube abnormality. We found a higher prevalence of
alterations in the tympanometry of mechanically ventilated
patients compared with previous studies. The etiology remains
to be defined, as a variety of factors play a role in the
Eustachian tube dysfunction.

The Impact of Extracellular miRNA let-7b as
a Top-Down Signal in the Auditory Brain
Ken Hayashi, MD, PhD (Presenter); Yuna Suzuki, MD;
Fumiyuki Goto, MD; Akihiro Kishino; Kaoru Ogawa, MD
Objectives: Our objective is to clarify the impact of extracellular let-7b as a top-down signal in auditory brain.
Methods: HEI-OC1 has been used as an auditory cell line.
Cell viability was determined using trypan blue. Western blot
analysis was performed on whole-cell extracts. Primary rat
cortex neuronal cell cultures were prepared from embryonic
day 18 Wister rat brains. The neural network was estimated on
the high-density CMOS-MEAs.
Results: The cell viability after direct treatment of let-7b
was time-dependently decreased. The expression of TLR7,
TRPA1, cleaved-caspase-3, LC3-II, and p-ERK were timedependently increased in let-7b-treated cells, whereas p62
was increased at the peak of 24 hours. The expression of TLR7
was decreased in TRPA1 KD cells, correlating with TRPA1.
These results indicate that TRL7 and TRPA1 form a complex,
enhanced in response to extracellular let-7b. However, the firing rate of primary neural cells was basically higher after
treatment of let-7b, whereas the extremely low firing rate cells
were also confirmed. This result suggests that the highfrequency firing rate cells have a possibility of cell death,
whereas the low-frequency firing rate cells have a possibility
of cellular hyperexcitability. The Fisher discrimination ratio
was significantly higher in control cells than let-7b-treated
cells. However, it divided into 2 types, the extremely high
group and extremely low group in let-7b-treated cells. This
result suggests that let-7b led to the neural cell dispersion and
disrupted the neural network.
Conclusions: Our results suggest that extracellular let-7b
influences hyperexcitability-induced cell death through the
interaction of TLR7 and TRPA1 as a top-down signal in auditory brain cells, disrupting the auditory neural network.
Implantation of Bone-Anchored Hearing Aid
Simultaneously With Chronic Otitis Media
Surgery: Complications and Benefits
Vanessa Pires Dinarte (Presenter); Ariele Pereira Macedo;
Laura Kracker Martucci; Vanessa Ota Nogueira;
Kamilla Paranagua Siqueira; Maressa Silveira Leal
Objectives: The bone-anchored hearing aid (BAHA) is an
effective form of hearing rehabilitation. Indications include
patients with single-side deafness, bilateral conductive, or
mixed permanent hearing loss. We evaluated the safety of the
implantation of concomitant BAHA implant with chronic otitis media (COM) and the presence of intra- or postoperative
complications.
Methods: A retrospective study was carried out with
6 patients who underwent BAHA implant simultaneously with
COM surgery, from March 2018 to December 2019, coming
from a private service in Marília, São Paulo, Brazil.



Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts

Table of Contents for the Digital Edition of Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts

Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts
Contents
Accreditation Information
2020 President Duane J. Taylor, MD
2020 Annual Meeting Program Committee
2020 Star Reviewers
2020 Distinguished Service Award Recipients
2020 Honor Award Recipients
Honorary Guest Lecturers 2020
Panel Presentations
Oral Presentations
Poster Presentations
Author Index
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Intro
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Cover2
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - A1
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - A2
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - A3
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Contents
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - A5
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - A6
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Accreditation Information
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - 2020 President Duane J. Taylor, MD
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - 2020 Annual Meeting Program Committee
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P4
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - 2020 Star Reviewers
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - 2020 Honor Award Recipients
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P7
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Honorary Guest Lecturers 2020
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P9
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Panel Presentations
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P11
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P12
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Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Oral Presentations
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Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P271
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P272
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P273
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P274
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P275
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P276
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P277
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P278
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P279
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P280
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P281
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P282
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P283
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P284
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P285
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P286
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P287
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P288
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P289
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P290
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P291
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P292
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P293
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P294
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P295
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P296
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P297
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P298
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P299
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P300
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P301
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P302
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P303
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P304
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P305
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P306
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P307
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P308
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P309
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P310
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P311
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P312
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Author Index
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P316
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P317
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P318
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P319
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P320
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P321
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P322
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P323
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P324
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P325
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P326
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P327
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P328
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P329
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P330
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P331
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - P332
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Cover3
Otolaryngology - Head & Neck Surgery Supplement - 2020 Official Program Abstracts - Cover4
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