Food Protection Trends - August 2009 - 534
Industry Products Fungal Toxin Mystery Solved Using Biolog’s Phenotype MicroArray™ Technology A Harvard Apparatus OEM Syringe Pump Modules from Harvard Apparatus H arvard Apparatus is pleased to offer its customers OEM Syringe Pump Modules to complete or customize their system. OEM Syringe Pump Modules include Microliter, Milliliter, Programmable and High Pressure Models. Alternatively, they can build a pump especially suited to the user’s research and design needs. Each OEM Syringe Pump features superior accuracy and precision as well as smooth flow. Customization of flow rate range, construction material, force, syringe size, control mechanism and much, much more are available. Harvard Apparatus is a global developer, manufacturer and distributor of innovative and specialized products to enhance bioresearch. Harvard Apparatus 800.272.2775 Holliston, MA www.harvardapparatus.com n important breakthrough in fungal toxin biology has been made possible through the use of Biolog’s Phenotype MicroArray technology. The fungus Fusarium graminearum is a major pathogen of wheat. It is the causative agent of head blight and results in substantial worldwide crop losses. Central to the infection process, the fungus produces a trichothecene toxin called deoxynivalenol. Although the toxin is produced at high levels during wheat infection, researchers have never been able to induce Fusarium to produce significant levels under laboratory culture conditions. This inability has hampered study of head blight disease and approaches to disease prevention. Now, this decades-old mystery has been solved. The CSIRO researchers used Biolog Phenotype MicroArray plates to culture the fungus simultaneously in hundreds of different micro-scale culture conditions. The set of culture conditions is designed to contain a diverse range of nutritional and stress conditions that a microbial cell might encounter. To facilitate detection, the CSIRO researchers constructed a special strain of the fungus, placing the green fluorescent protein under the genetic control region for the first step in the toxin synthesis pathway. If any culture condition induced synthesis of the toxin gene, the cells in that micro-well would exhibit green fluorescence. In a single experiment, they quickly screened hundreds of conditions and determined that key factors for turning on toxin synthesis are the presence of specific nitrogen compounds (arginine, agmatine, putrescine) as well as low extracellular pH. Even more generally, this work demonstrates the feasibility of using Biolog Phenotype MicroArray technology to study the induction of any microbially produced toxin or secondary metabolite. Toxins, and other secondary metabolites such as bacteriocins and other antibiotic compounds are typically produced under some special culture conditions that are difficult to determine. Biolog’s technology can provide breakthrough discovery in a single experiment. The utility is further demonstrated in a commercial application in a recent publication from Wyeth Research (Maya Singh, Journal of Microbiological Methods 77:102) who found culture conditions for high levels of antibacterial chemical production by fungi. Data from Phenotype MicroArray experiments have also contributed to the realization that intracellular hexose phosphates are chemical signals that turn on production of the toxin listeriolysin O during human infections by the bacterium Listeria monocytogenes (Barry Bochner, FEMS Microbiology Reviews 33:191). Phenotype MicroArray technology, developed with SBIR funding from NIH, is more and more proving to be an important breakthrough technology. It allows scientists to study the growth properties and Be sure to mention, “I read about it in Food Protection Trends”! The publishers do not warrant, either expressly or by implication, the factual accuracy of the products or descriptions herein, nor do they so warrant any views or opinions offered by the manufacturer of said articles and products. 534 FOOD PROTECTION TRENDS | AUGUST 2009
http://www.harvardapparatus.com
Food Protection Trends - August 2009
Table of Contents for the Digital Edition of Food Protection Trends - August 2009
Food Protection - August 2009
Contents
Sustaining Members
Vickie’s View from Your President
Commentary from the Executive Director
Contamination Prevention and Response Related to Fresh and Fresh-cut Produce: An Expert Perspective on the Farmer Decision Making Process
Control of Salmonella in Low-moisture Foods III: Process Validation and Environmental Monitoring–Part three of a three-part series
Audiovisual Library Listing
Audiovisual Library Order Form
New Members
What’s Happening in Food Safety
Industry Products
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Booklet Order Form
Membership Application
Food Protection Trends - August 2009 - Food Protection - August 2009
Food Protection Trends - August 2009 - Cover2
Food Protection Trends - August 2009 - 473
Food Protection Trends - August 2009 - Contents
Food Protection Trends - August 2009 - 475
Food Protection Trends - August 2009 - 476
Food Protection Trends - August 2009 - 477
Food Protection Trends - August 2009 - 478
Food Protection Trends - August 2009 - 479
Food Protection Trends - August 2009 - 480
Food Protection Trends - August 2009 - Sustaining Members
Food Protection Trends - August 2009 - 482
Food Protection Trends - August 2009 - 483
Food Protection Trends - August 2009 - Vickie’s View from Your President
Food Protection Trends - August 2009 - 485
Food Protection Trends - August 2009 - Commentary from the Executive Director
Food Protection Trends - August 2009 - 487
Food Protection Trends - August 2009 - Contamination Prevention and Response Related to Fresh and Fresh-cut Produce: An Expert Perspective on the Farmer Decision Making Process
Food Protection Trends - August 2009 - 489
Food Protection Trends - August 2009 - 490
Food Protection Trends - August 2009 - 491
Food Protection Trends - August 2009 - 492
Food Protection Trends - August 2009 - Control of Salmonella in Low-moisture Foods III: Process Validation and Environmental Monitoring–Part three of a three-part series
Food Protection Trends - August 2009 - 494
Food Protection Trends - August 2009 - 495
Food Protection Trends - August 2009 - 496
Food Protection Trends - August 2009 - 497
Food Protection Trends - August 2009 - 498
Food Protection Trends - August 2009 - 499
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Food Protection Trends - August 2009 - 506
Food Protection Trends - August 2009 - 507
Food Protection Trends - August 2009 - 508
Food Protection Trends - August 2009 - 509
Food Protection Trends - August 2009 - Audiovisual Library Listing
Food Protection Trends - August 2009 - 511
Food Protection Trends - August 2009 - 512
Food Protection Trends - August 2009 - 513
Food Protection Trends - August 2009 - 514
Food Protection Trends - August 2009 - 515
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Food Protection Trends - August 2009 - 521
Food Protection Trends - August 2009 - 522
Food Protection Trends - August 2009 - 523
Food Protection Trends - August 2009 - 524
Food Protection Trends - August 2009 - Audiovisual Library Order Form
Food Protection Trends - August 2009 - New Members
Food Protection Trends - August 2009 - 527
Food Protection Trends - August 2009 - 528
Food Protection Trends - August 2009 - 529
Food Protection Trends - August 2009 - What’s Happening in Food Safety
Food Protection Trends - August 2009 - 531
Food Protection Trends - August 2009 - 532
Food Protection Trends - August 2009 - 533
Food Protection Trends - August 2009 - Industry Products
Food Protection Trends - August 2009 - 535
Food Protection Trends - August 2009 - 536
Food Protection Trends - August 2009 - 537
Food Protection Trends - August 2009 - 538
Food Protection Trends - August 2009 - 539
Food Protection Trends - August 2009 - Coming Events
Food Protection Trends - August 2009 - 541
Food Protection Trends - August 2009 - Advertising Index
Food Protection Trends - August 2009 - 543
Food Protection Trends - August 2009 - 544
Food Protection Trends - August 2009 - 545
Food Protection Trends - August 2009 - Journal of Food Protection Table of Contents
Food Protection Trends - August 2009 - Booklet Order Form
Food Protection Trends - August 2009 - Membership Application
Food Protection Trends - August 2009 - Cover3
Food Protection Trends - August 2009 - Cover4
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