Food Protection Trends - May 2010 - 273
Copyright© 2010, International Association for Food Protection 6200 Aurora Ave., Suite 200W, Des Moines, IA 50322-2864
Food Protection Trends, Vol. 30, No. 5, Pages 273–279
Characterization of Multidrugresistant Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen and Typhimurium Isolated from Feedlot Cattle
EbOT S.TAbE,1 JAMES OlOYA,2 DAwN K. DOETKOTT1 and MARgARET l. KhAITSA1* 1 Dept. of Veterinary and Microbiological Sciences, North Dakota State University, 1523 Centennial blvd., Fargo, ND 58105–5406, USA; 2Dept. of Epidemiology and biostatistics, University of georgia, 235b Paul D. Coverdell Center, Athens, gA 30602–0001, USA
ABSTRACT The objective of this study was to characterize Salmonella enterica subsp. enterica Serovars Typhimurium and Typhimurium var. Copenhagen isolated from naturally infected feedlot cattle (n = 138) in North Dakota for antimicrobial resistance (AMR), presence of integrons and genotypic relatedness by use of PFgE assays. A panel of 15 selected antimicrobials and the Sensititre automated antimicrobial susceptibility test system (TREK Diagnostic Systems, westlake, Oh) were used. Class 1 and 2 integrons were targeted by PCR, using primers specific for the intI1 and intI2 genes. Pulsed field gel electrophoresis (PFgE) assays were performed by the E. coli Reference Center, Pennsylvania State University, University Park, PA. All 58 Salmonella isolates tested were resistant to ≥ 1 of the antimicrobials, with 56/58 (96.6%) showing multidrug resistance (resistant to ≥ 2 antimicrobials). Twenty-nine (26 of which were Salmonella serovars Typhimurium var Copenhagen) were positive for class I integron; two also tested positive for integron 2. The 58 Salmonella isolates were grouped into 9 distinguishable PFgE profiles, with the most prevalent genotype accounting for 46.6% (27/58) of the isolates. The predominant resistance phenotype (94.8%, 55/58) was Amox/Cla-Amp-Chl-StrSul-Tet. In addition, these data indicate that Salmonella enterica subsp. enterica Serovars Typhimurium and Typhimurium var. Copenhagen Salmonella isolated from naturally infected feedlot cattle in North Dakota showed widespread AMR, with or without presence of class 1 integron.
A peer-reviewed article
* Author for correspondence: Phone: 701.231.5946; Fax: 701.231.7514 E-mail: Margaret.Khaitsa@ndsu.edu
MAY 2010 | FOOD PROTECTION TRENDS
273
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http://www.foodprotection.org/files/food-protection-trends/May-10-Khaitsa.pdf
Food Protection Trends - May 2010
Table of Contents for the Digital Edition of Food Protection Trends - May 2010
Food Protection Trends - May 2010
Contents
Sustaining Members
Vickie’s View from Your President
Commentary from the Executive Director
Use of Linear Models for Thermal Processing of Acidified Foods
Characterization of Multidrug-resistant Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen and Typhimurium Isolated from Feedlot Cattle
IAFP Secretary Announcement
Highlights from the Dubai International Food Safety Conference
New Members
What’s Happening in Food Safety
Industry Products
Preliminary Program
Ivan Parkin Lecture
John H. Silliker Lecture
Activities
General Information
Exhibitors
Workshops
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Audiovisual Library Order Form
Booklet Order Form
Membership Application
Food Protection Trends - May 2010 - Food Protection Trends - May 2010
Food Protection Trends - May 2010 - Cover2
Food Protection Trends - May 2010 - 253
Food Protection Trends - May 2010 - Contents
Food Protection Trends - May 2010 - 255
Food Protection Trends - May 2010 - 256
Food Protection Trends - May 2010 - 257
Food Protection Trends - May 2010 - 258
Food Protection Trends - May 2010 - 259
Food Protection Trends - May 2010 - 260
Food Protection Trends - May 2010 - Sustaining Members
Food Protection Trends - May 2010 - 262
Food Protection Trends - May 2010 - 263
Food Protection Trends - May 2010 - Vickie’s View from Your President
Food Protection Trends - May 2010 - 265
Food Protection Trends - May 2010 - Commentary from the Executive Director
Food Protection Trends - May 2010 - 267
Food Protection Trends - May 2010 - Use of Linear Models for Thermal Processing of Acidified Foods
Food Protection Trends - May 2010 - 269
Food Protection Trends - May 2010 - 270
Food Protection Trends - May 2010 - 271
Food Protection Trends - May 2010 - 272
Food Protection Trends - May 2010 - Characterization of Multidrug-resistant Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen and Typhimurium Isolated from Feedlot Cattle
Food Protection Trends - May 2010 - 274
Food Protection Trends - May 2010 - 275
Food Protection Trends - May 2010 - 276
Food Protection Trends - May 2010 - 277
Food Protection Trends - May 2010 - 278
Food Protection Trends - May 2010 - 279
Food Protection Trends - May 2010 - 280
Food Protection Trends - May 2010 - IAFP Secretary Announcement
Food Protection Trends - May 2010 - Highlights from the Dubai International Food Safety Conference
Food Protection Trends - May 2010 - 283
Food Protection Trends - May 2010 - 284
Food Protection Trends - May 2010 - 285
Food Protection Trends - May 2010 - New Members
Food Protection Trends - May 2010 - 287
Food Protection Trends - May 2010 - 288
Food Protection Trends - May 2010 - What’s Happening in Food Safety
Food Protection Trends - May 2010 - 290
Food Protection Trends - May 2010 - 291
Food Protection Trends - May 2010 - 292
Food Protection Trends - May 2010 - Industry Products
Food Protection Trends - May 2010 - 294
Food Protection Trends - May 2010 - 295
Food Protection Trends - May 2010 - 296
Food Protection Trends - May 2010 - 297
Food Protection Trends - May 2010 - 298
Food Protection Trends - May 2010 - 299
Food Protection Trends - May 2010 - 300
Food Protection Trends - May 2010 - Preliminary Program
Food Protection Trends - May 2010 - Ivan Parkin Lecture
Food Protection Trends - May 2010 - John H. Silliker Lecture
Food Protection Trends - May 2010 - Activities
Food Protection Trends - May 2010 - General Information
Food Protection Trends - May 2010 - Exhibitors
Food Protection Trends - May 2010 - 307
Food Protection Trends - May 2010 - Workshops
Food Protection Trends - May 2010 - 309
Food Protection Trends - May 2010 - 310
Food Protection Trends - May 2010 - 311
Food Protection Trends - May 2010 - Coming Events
Food Protection Trends - May 2010 - 313
Food Protection Trends - May 2010 - Advertising Index
Food Protection Trends - May 2010 - 315
Food Protection Trends - May 2010 - Journal of Food Protection Table of Contents
Food Protection Trends - May 2010 - 317
Food Protection Trends - May 2010 - Audiovisual Library Order Form
Food Protection Trends - May 2010 - Booklet Order Form
Food Protection Trends - May 2010 - Membership Application
Food Protection Trends - May 2010 - Cover3
Food Protection Trends - May 2010 - Cover4
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