Food Protection Trends - May 2010 - 276
FIGURE 1. Dendrogram generated from the Xbal patterns of the 58 Salmonella isolates using UPGMA clustering analysis with the BioNumerics software. A positive tolerance of 1.5% was chosen.
RESULTS
Salmonella serotypes and antimicrobial susceptibility testing
A total of 54 of 58 (93.1%) of the Salmonella species belonged to the serotype Typhimurium vars Copenhagen. The rest (4/58, 6.9%) were Salmonella Typhimurium. AMR testing showed that all isolates were resistant to more than one of the antibiotics (Table 1). All but two of the isolates (ID 26 and 30) were resistant to more than two of the antibiotics tested, with 96.6% (56 of 58) of the isolates being MDR. All isolates tested were susceptible to amikacin, cefoxitin, cetriaxone, ciprofloxacin, gentamycin, nalidixic acid, and trimethoprim-sulfamethoxazole. Almost all of the isolates recovered from this study had a similar antimicrobial resistance pattern.
isolates showed a 233-bp PCR product by use of primers intI2, suggesting the presence of class 2 integron. Both of the two isolates also carried the class 1 integron.
respectively. The relationship between molecular types, integron carriage and resistance phenotypes of the Salmonella Typhimurium serovars Copenhagen is shown in Table 2.
PFGE analysis
The PFGE analysis identified 9 distinguishable Salmonella genotypes. Of the 9 PFGE profiles, STC and ST accounted for 94.8% (55 of 58) and 5.2 % (3 of 58) of the isolates, respectively (Fig. 1). Type IV, V, VII, VIII, and IX derived from cattle at two sampling periods showed 100% similarity. Type X (1 isolate from sampling 2), which is the most distant strain, showed only 73% similarity in PFGE banding patterns. Genotype V was the most prevalent (28/58, 48.3%) of the isolates, followed by types VI (15/58, 25.9%), IV and IX (both 3/58, 5.2%). Additionally, genotypes I, II, II, and X each represented 1.7% (1 of 58) of the genotypes (Fig. 1). Isolates 49 and 65, which were positive for both Int 1 and 2, belonged to genotypes I and IV,
DISCUSSION
In this study, all but two of the Salmonella isolates were resistant to more than two of the antimicrobials tested, with 96.6% of the isolates showing multidrug resistance. The widespread AMR of Salmonella isolated from cattle in North Dakota has been reported before (23), with most animal strains showing more multidrug resistance than human Salmonella isolates show, possibly due to a difference in antimicrobial selection pressure exerted on the microorganisms in the two populations. The emergence and dissemination of MDR among Salmonella isolates from healthy cattle have potential adverse implications to public health. Since the first description of class 1 integron by
Presence of integrons
Regardless of sampling points (1, 2, or 3), 29 were positive for class I integron (280 bp product) while only two of the
276 FOOD PROTECTION TRENDS | MAY 2010
Food Protection Trends - May 2010
Table of Contents for the Digital Edition of Food Protection Trends - May 2010
Food Protection Trends - May 2010
Contents
Sustaining Members
Vickie’s View from Your President
Commentary from the Executive Director
Use of Linear Models for Thermal Processing of Acidified Foods
Characterization of Multidrug-resistant Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen and Typhimurium Isolated from Feedlot Cattle
IAFP Secretary Announcement
Highlights from the Dubai International Food Safety Conference
New Members
What’s Happening in Food Safety
Industry Products
Preliminary Program
Ivan Parkin Lecture
John H. Silliker Lecture
Activities
General Information
Exhibitors
Workshops
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Audiovisual Library Order Form
Booklet Order Form
Membership Application
Food Protection Trends - May 2010 - Food Protection Trends - May 2010
Food Protection Trends - May 2010 - Cover2
Food Protection Trends - May 2010 - 253
Food Protection Trends - May 2010 - Contents
Food Protection Trends - May 2010 - 255
Food Protection Trends - May 2010 - 256
Food Protection Trends - May 2010 - 257
Food Protection Trends - May 2010 - 258
Food Protection Trends - May 2010 - 259
Food Protection Trends - May 2010 - 260
Food Protection Trends - May 2010 - Sustaining Members
Food Protection Trends - May 2010 - 262
Food Protection Trends - May 2010 - 263
Food Protection Trends - May 2010 - Vickie’s View from Your President
Food Protection Trends - May 2010 - 265
Food Protection Trends - May 2010 - Commentary from the Executive Director
Food Protection Trends - May 2010 - 267
Food Protection Trends - May 2010 - Use of Linear Models for Thermal Processing of Acidified Foods
Food Protection Trends - May 2010 - 269
Food Protection Trends - May 2010 - 270
Food Protection Trends - May 2010 - 271
Food Protection Trends - May 2010 - 272
Food Protection Trends - May 2010 - Characterization of Multidrug-resistant Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen and Typhimurium Isolated from Feedlot Cattle
Food Protection Trends - May 2010 - 274
Food Protection Trends - May 2010 - 275
Food Protection Trends - May 2010 - 276
Food Protection Trends - May 2010 - 277
Food Protection Trends - May 2010 - 278
Food Protection Trends - May 2010 - 279
Food Protection Trends - May 2010 - 280
Food Protection Trends - May 2010 - IAFP Secretary Announcement
Food Protection Trends - May 2010 - Highlights from the Dubai International Food Safety Conference
Food Protection Trends - May 2010 - 283
Food Protection Trends - May 2010 - 284
Food Protection Trends - May 2010 - 285
Food Protection Trends - May 2010 - New Members
Food Protection Trends - May 2010 - 287
Food Protection Trends - May 2010 - 288
Food Protection Trends - May 2010 - What’s Happening in Food Safety
Food Protection Trends - May 2010 - 290
Food Protection Trends - May 2010 - 291
Food Protection Trends - May 2010 - 292
Food Protection Trends - May 2010 - Industry Products
Food Protection Trends - May 2010 - 294
Food Protection Trends - May 2010 - 295
Food Protection Trends - May 2010 - 296
Food Protection Trends - May 2010 - 297
Food Protection Trends - May 2010 - 298
Food Protection Trends - May 2010 - 299
Food Protection Trends - May 2010 - 300
Food Protection Trends - May 2010 - Preliminary Program
Food Protection Trends - May 2010 - Ivan Parkin Lecture
Food Protection Trends - May 2010 - John H. Silliker Lecture
Food Protection Trends - May 2010 - Activities
Food Protection Trends - May 2010 - General Information
Food Protection Trends - May 2010 - Exhibitors
Food Protection Trends - May 2010 - 307
Food Protection Trends - May 2010 - Workshops
Food Protection Trends - May 2010 - 309
Food Protection Trends - May 2010 - 310
Food Protection Trends - May 2010 - 311
Food Protection Trends - May 2010 - Coming Events
Food Protection Trends - May 2010 - 313
Food Protection Trends - May 2010 - Advertising Index
Food Protection Trends - May 2010 - 315
Food Protection Trends - May 2010 - Journal of Food Protection Table of Contents
Food Protection Trends - May 2010 - 317
Food Protection Trends - May 2010 - Audiovisual Library Order Form
Food Protection Trends - May 2010 - Booklet Order Form
Food Protection Trends - May 2010 - Membership Application
Food Protection Trends - May 2010 - Cover3
Food Protection Trends - May 2010 - Cover4
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