Food Protection Trends - May/June 2023 - 279

TABLE 5. Characteristics of common test methods
Characteristic
rt-PCR
Specificity based upon (10)
Limit of detection (CFU/mL)
Typical TAT for Salmonella
and Listeria
DNA or RNA sequences
102
-104
(17)
1 day
PCR
ELISA
DNA or RNA sequences
(17)
102-105
104
(11)
1-2 days
Antibody-antigen binding
(17)
102-105
104-105
(11)
2 days
TABLE 6. FDA equivalent rapid methods based on validation accreditation and reference
method only
Example
1
2
3
4
5
6
Test
Rapid PCR test
Rapid ELISA test
Rapid PCR test
Rapid ELISA test
Rapid PCR test
Rapid ELISA test
Rapid PCR test
Rapid ELISA test
Rapid PCR test
Rapid ELISA test
Rapid PCR test
Rapid ELISA test
Validation protocol
AOAC
AOAC
AOAC
AFNOR
AOAC
AFNOR
AOAC
AOAC
AFNOR
AFNOR
AFNOR
AFNOR
among methods, the validated protocol for the matrix results
in an equivalent probability for detection. DeMarco et al. (7)
studied the effects of different lysis procedures and volumes
delivered to the final molecular detection reaction when
comparing PCR, rt-PCR, and loop-mediated amplification
methods. In doing so, they observed differences in sensitivity
among the detection technologies but concluded that this
finding would not be expected to impact reported results.
The methods were performed using their validated protocol
and were all sensitive to the level claimed in their validated
protocol.
FINDING EQUIVALENT METHODS
In the examples above, the methods to be compared were
already known. In circumstances for which an alternative
method must be found, the critical criteria are the test-portion
size(s) and target analyte(s). An approach such as that outlined
Reference method
FDA BAM
FDA BAM
FDA BAM
FDA BAM
FDA BAM
ISO
FDA BAM
ISO
FDA BAM
FDA BAM
ISO
ISO
Equivalent
Yes
Yes
No
No
Yes
Yes-but reference method
not recognized by FDA
in Table 6 could be used to find an equivalent rapid method
that has certifications equivalent for the intended use, though
compromises may need to be made on TAT, ease of use, or
cost. Looking at example 1 outlined in Table 6 we see that if
comparing a rapid PCR method against a rapid enzyme-linked
immunosorbent assay (ELISA) method, where both are
validated or certified for the same matrix and test portion
size against an FDA BAM reference method according to the
AOAC Appendix J protocol, we can consider them equivalent.
In example 2, with different validation protocols but the same
reference method, we can also consider these methods equivalent.
Conversely, looking at example 3, we see that if the same
two methods were validated or certified against different reference
methods we cannot consider them equivalent without
doing some additional comparisons. Table 6 outlines interpretations
for additional permutations of validation conditions to
assist with a range of commonly encountered decisions.
May/June Food Protection Trends 279

Food Protection Trends - May/June 2023

Table of Contents for the Digital Edition of Food Protection Trends - May/June 2023

Food Allergy Knowledge and Attitudes of Owners and Managers of Independently Owned Restaurants in Metro Orlando, Florida
Face Masks as Sources of Cross-Contamination during Food Preparation
Food Safety Knowledge and Practices among Household Food Handlers in Mangweni Village of Mpumalanga Province in South Africa
Risk Management Options to Reduce Human Salmonellosis Cases Due to Consumption of Raw Poultry
Beyond the Bio Almaris Alonso-Claudio
PDG Highlight Viral and Parasitic Foodborne Disease PDG
General Interest Evaluating Microbiological Method Equivalence – A Decision Guide
Industry Products
Coming Events
Food Protection Trends - May/June 2023 - Cover1
Food Protection Trends - May/June 2023 - Cover2
Food Protection Trends - May/June 2023 - 197
Food Protection Trends - May/June 2023 - 198
Food Protection Trends - May/June 2023 - 199
Food Protection Trends - May/June 2023 - 200
Food Protection Trends - May/June 2023 - 201
Food Protection Trends - May/June 2023 - 202
Food Protection Trends - May/June 2023 - 203
Food Protection Trends - May/June 2023 - Food Allergy Knowledge and Attitudes of Owners and Managers of Independently Owned Restaurants in Metro Orlando, Florida
Food Protection Trends - May/June 2023 - 205
Food Protection Trends - May/June 2023 - 206
Food Protection Trends - May/June 2023 - 207
Food Protection Trends - May/June 2023 - 208
Food Protection Trends - May/June 2023 - 209
Food Protection Trends - May/June 2023 - 210
Food Protection Trends - May/June 2023 - 211
Food Protection Trends - May/June 2023 - 212
Food Protection Trends - May/June 2023 - 213
Food Protection Trends - May/June 2023 - 214
Food Protection Trends - May/June 2023 - Face Masks as Sources of Cross-Contamination during Food Preparation
Food Protection Trends - May/June 2023 - 216
Food Protection Trends - May/June 2023 - 217
Food Protection Trends - May/June 2023 - 218
Food Protection Trends - May/June 2023 - 219
Food Protection Trends - May/June 2023 - 220
Food Protection Trends - May/June 2023 - 221
Food Protection Trends - May/June 2023 - 222
Food Protection Trends - May/June 2023 - Food Safety Knowledge and Practices among Household Food Handlers in Mangweni Village of Mpumalanga Province in South Africa
Food Protection Trends - May/June 2023 - 224
Food Protection Trends - May/June 2023 - 225
Food Protection Trends - May/June 2023 - 226
Food Protection Trends - May/June 2023 - 227
Food Protection Trends - May/June 2023 - 228
Food Protection Trends - May/June 2023 - 229
Food Protection Trends - May/June 2023 - 230
Food Protection Trends - May/June 2023 - 231
Food Protection Trends - May/June 2023 - Risk Management Options to Reduce Human Salmonellosis Cases Due to Consumption of Raw Poultry
Food Protection Trends - May/June 2023 - 233
Food Protection Trends - May/June 2023 - 234
Food Protection Trends - May/June 2023 - 235
Food Protection Trends - May/June 2023 - 236
Food Protection Trends - May/June 2023 - 237
Food Protection Trends - May/June 2023 - 238
Food Protection Trends - May/June 2023 - 239
Food Protection Trends - May/June 2023 - Beyond the Bio Almaris Alonso-Claudio
Food Protection Trends - May/June 2023 - 241
Food Protection Trends - May/June 2023 - 242
Food Protection Trends - May/June 2023 - PDG Highlight Viral and Parasitic Foodborne Disease PDG
Food Protection Trends - May/June 2023 - 244
Food Protection Trends - May/June 2023 - 245
Food Protection Trends - May/June 2023 - 246
Food Protection Trends - May/June 2023 - 247
Food Protection Trends - May/June 2023 - 248
Food Protection Trends - May/June 2023 - 249
Food Protection Trends - May/June 2023 - 250
Food Protection Trends - May/June 2023 - 251
Food Protection Trends - May/June 2023 - 252
Food Protection Trends - May/June 2023 - 253
Food Protection Trends - May/June 2023 - 254
Food Protection Trends - May/June 2023 - 255
Food Protection Trends - May/June 2023 - 256
Food Protection Trends - May/June 2023 - 257
Food Protection Trends - May/June 2023 - 258
Food Protection Trends - May/June 2023 - 259
Food Protection Trends - May/June 2023 - 260
Food Protection Trends - May/June 2023 - 261
Food Protection Trends - May/June 2023 - 262
Food Protection Trends - May/June 2023 - 263
Food Protection Trends - May/June 2023 - 264
Food Protection Trends - May/June 2023 - 265
Food Protection Trends - May/June 2023 - 266
Food Protection Trends - May/June 2023 - 267
Food Protection Trends - May/June 2023 - 268
Food Protection Trends - May/June 2023 - 269
Food Protection Trends - May/June 2023 - 270
Food Protection Trends - May/June 2023 - 271
Food Protection Trends - May/June 2023 - 272
Food Protection Trends - May/June 2023 - 273
Food Protection Trends - May/June 2023 - 274
Food Protection Trends - May/June 2023 - General Interest Evaluating Microbiological Method Equivalence – A Decision Guide
Food Protection Trends - May/June 2023 - 276
Food Protection Trends - May/June 2023 - 277
Food Protection Trends - May/June 2023 - 278
Food Protection Trends - May/June 2023 - 279
Food Protection Trends - May/June 2023 - 280
Food Protection Trends - May/June 2023 - Industry Products
Food Protection Trends - May/June 2023 - 282
Food Protection Trends - May/June 2023 - 283
Food Protection Trends - May/June 2023 - Coming Events
Food Protection Trends - May/June 2023 - Cover3
Food Protection Trends - May/June 2023 - Cover4
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