IAFP 2009 Program Book and Abstract Book - AB128

Methods: At each location, 10 Clean-Trace Surface ATP swabs and 10 swabs from the manufacturing plant’s ATP system were dosed with 25 microliters of and ATP solution. The swabs were activated and measured as per the manufacturer’s directions and the results recorded in a spreadsheet. The Coefficient of Variation (CV) was calculated for each system by dividing the standard deviation of the measurements by the mean. Results: The results were analyzed on the basis of individual head-to-head comparisons and in aggregate, by averaging the CV’s determined in each individual comparison. The Clean-Trace ATP system had a lower CV in every comparison. In aggregate, the systems performed as follows: Biocontrol CV = 45.95% (n = 90), Charm CV = 34.16% (n = 280), Hygiena CV = 35.74% (n = 180), Neogen CV = 94.05% (n = 160), 3M CV = 12.11% (n = 660). Significance: The repeatability of ATP Testing Systems has direct quality and financial implications for the food processor. Both immediate decisions (re-clean or release for production) and long term decisions in the plant are based on data from ATP testing systems, and these decisions may be faulty if based on faulty data, ultimately resulting in financial loss and increased risk. P2-66 Removal Effects of Electrolyzed Water against Bacterial Biofilms KYU-DUCK CHOI, Yaru Quan, Dong-Hwa Chung and Il-Shik Shin Kangnung National University, Faculty of Marine Bioscience and Technology, Gangneung City, Korea, South Introduction: Microbial biofilms are more resistant to sanitizers, disinfectants and antimicrobial agents than planktonic cells; even with cleaning and sanitation procedures, bacteria were found on equipment surfaces. Thus, the biofilms in food processing facilities need more effective cleaning and sanitation procedures. Although electrolyzed water (EW) has been proved to have strong antimicrobial activities against most pathogenic bacteria, research on the effects of electrolyzed water on bacterial biofilms is rare. Purpose: The purpose of this study was to evaluate the effects of strong acidic electrolyzed water (SEW) and weak acidic electrolyzed water (WEW) on Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 and Vibrio vulnificus biofilms. Methods: Each 2 mL of cell culture (108 CFU/mL) of L. monocytogenes ATCC 15313, S. aureus ATCC 12692, E. coli O157:H7 ATCC 25922 and V. vulnificus ATCC 27562 was separately added to a tube containing 25 mL of fresh broth (tryptic soy broth for L. monocytogenes, S. aureus and E. coli O157:H7, and brain heart infusion broth with 1% NaCl for V. vulnificus) into which one stainless steel (SS) chip was immersed. The biofilm was allowed to develop on the SS at 37°C for 8 days. Three methods were used to remove adherent cells from SS by SEW and WEW: swabbing (a pre-moistened swab in PBS for 20 times per side), scraping with a Teflon spatula, followed by swabbing; and ultrasonic washing (sonicated in ultrasonic washer for 3 min and then vortexed for 1 min). The biofilms of four bacterial strains on stainless steel (SS) type 304 surface were treated by 10 ppm to 50 ppm of available chlorine concentration of SEW or WEW for 15 s to 10 min. Results: Submersion of stainless steel chip attached with bacterial biofilm in SEW (50 ppm of available chlorine concentration) within 5 min reduced the cell counts from 5.9 to 2.1 log CFU/cm2 for V. vulnificus, from 6.7 to 1.0 log CFU/ cm2 for E. coli O157:H7, from 5.4 to 3.2 log CFU/cm2 for L. monocytogenes, and from 4.9 to 3.2 log CFU/cm2 for S. aureus. When treatment time was increased to 10 min, 50 ppm of SEW could completely eliminate L. monocytogenes and S. aureus biofilm. Almost identical results were obtained from WEW treatments. Significance: SEW and WEW could effectively kill viable cells in biofilms and eliminate the bacterial secretion (extracellular polymeric substances) of bacteria on a surface and obviously reduced the available chlorine concentration and treatment time compared with a chlorine solution. Thus, SEW or WEW is a potential disinfectant agent in food processing facilities. P2-67 Control of Listeria monocytogenes on Contact and Non-contact Surfaces by Electrostatic Spraying of Quaternary DSC ANDREA E. DOW, Christine Alvarado, Mindy Brashears and Pawan Takhar Texas Tech University, Animal and Food Sciences, Lubbock, TX, USA Introduction: The attachment of bacteria on food processing equipment and in the environment after sanitation can cause potential cross-contamination, which can lead to surface destruction, food spoilage, and possible food safety concerns. Purpose: The purpose of this research was to determine if electrostatic spraying of quaternary ammonium compounds would provide a more efficient means of sanitizing food contact and environmental surfaces to reduce bacterial attachment and prevent biofilm formation. Methods: Ceramic tile, FRP (plastic wall board), polypropylene conveyor belt-mesh top (24% open mesh) and stainless steel conveyor – single loop (80% open mesh) were inoculated with a Listeria monocytogenes (LM) cocktail with a final concentration of 106 CFU/ ml and then subjected to either an air pressure spray or an electrostatic spray treatment using 200 ppm of ala-quaternary ammonium. Results: There were significant (P < 0.05) reductions in the amount of LM that remained on the surfaces after being treated with both the electrostatic spray and the air-pressure spray, but no significant differences between the two treatments (P < 0.05). To determine which treatment could prevent biofilm formation, the ceramic tile, FRP stainless , steel coupons (306 food grade), and polyethylene (plastic cutting board) were treated with either an air pressure spray or an electrostatic spray treatment using 200 ppm of ala-quaternary ammonium. Biofilms were allowed to form onto the surfaces for 24 h. The biofilms were measured by crystal method analysis and scanning electron microscopy (SEM). The crystal method analysis indicated that electrostatic spray significantly (P < 0.05) reduced the biofilm formation on all the surfaces, and the SEM confirmed the absorbency reading. 128 — Abstract Book Poster

IAFP 2009 Program Book and Abstract Book

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