IAFP 2009 Program Book and Abstract Book - AB187

Results: Infectious MNV-1 was not recovered from the stainless steel coupons (10 PFU/ml detection limit) whether integrated simultaneously or after 6 h of biofilm formation. The bacterial population recovered from the coupons was approximately 103 CFU/ml except after disinfectant treatment, from which < 10 CFU/ml were recovered. Significance: Detection of infective MNV-1 from 48-h biofilms on stainless steel was not possible with the low inoculum and recovery methods employed. Current investigations include evaluation of higher initial virus populations and different recovery and detection methods on determination of virus integration and survival in bacterial biofilms. P3-106 Antimicrobial Activities of Cinnamaldehyde and Carvacrol against Salmonella Newport on Contaminated Oyster and Celery SADHANA RAVISHANKAR, Libin Zhu and Mendel Friedman University of Arizona, Veterinary Science & Microbiology, Tucson, AZ, USA Introduction: Consumers generally prefer natural over synthetic food additives. Safe plant extracts such as essential oils, their active components, and spices have been shown to exhibit antimicrobial effects against foodborne pathogens. Purpose: The objective of this study was to define the antimicrobial activities of cinnamaldehyde (the main ingredient of cinnamon oil) and carvacrol (the main ingredient of oregano oil), against Salmonella Newport on oysters and celery. Methods: Oyster or celery samples were dipped in S. Newport culture (107 CFU/ml) for 2 min, and dried for 1 h. Celery samples were dipped in 1% cinnamaldehyde or carvacrol in phosphate buffered saline (PBS) for 10 min and oyster samples for 10 min or 1 h. All samples were stored at 4°C for 3 days. Enumerations of surviving bacteria were done at days 0 and 3. Results: When samples were dipped in 1% carvacrol for 10 min, no survivors were detected on celery at day 0. There were about 1 and 1.5 log reductions on oysters at days 0 and 3, respectively. Oyster samples dipped in carvacrol for 1 hr showed about 1.5 and 5 log reductions at days 0 and day 3, respectively. Celery samples exposed to cinnamaldehyde showed 1 and 2.5 log reductions at days 0 and 3, respectively. The bacterial count on oysters exposed for 10 min to cinnamaldehyde was reduced by about 1 and 2 logs at days 0 and 3, respectively. Oysters exposed for 1 hr to cinnamaldehyde had 1 and 5 log reductions at days 0 and 3, respectively. Dipping test samples in PBS (control) had no effect on Salmonella. Significance: This study demonstrates the potential of carvacrol and cinnamaldehyde to inactivate S. Newport on contaminated celery and oysters. The potential of these compounds for inactivation of S. Newport and other pathogens on produce and seafood merits study by the food industry. P3-107 Risk Factors Associated with the Presence of Listeria in Rural Households with or without Ruminant Animals MAWILL RODRIGUEZ-MARVAL, Jeff LeJeune, Lydia C. Medeiros, Patricia A. Kendall and John N. Sofos Colorado State University, Dept. of Animal Sciences, Fort Collins, CO, USA Introduction: Ruminants may be a reservoir for Listeria and a potential source of household contamination. However, there are no available data on how Listeria could be introduced in the household environment. Understanding consumer behavior may help in development of education materials on preventive measures to further reduce the risk of listeriosis. Purpose: This study evaluated consumer behaviors in households with or without ruminants, associated with cleaning, food handling and storage, which may be related to increased prevalence of Listeria in the household environment. Methods: Rural Colorado households (27 with and 27 without ruminants) were recruited, samples (food, environmental, and human and animal feces) were collected four times (at 2 to 3 week intervals), and tested for Listeria. Participants answered surveys regarding cleaning habits, food handling (storage, preparation and preferences), and animal handling. The study was completed over a three-year period, with samples collected during years one and three. Seven indices were developed from survey information, and were statistically analyzed for relationships to the outcome with a sample positive for Listeria as the dependent variable. Results: Listeria was isolated from all types of samples with higher prevalence in households with ruminants. Compared to year-1, prevalence in food, refrigerators and washing machines was higher in year-3, and prevalence on kitchen sinks, shoes, working gloves and ruminant feces was lower. The perishable food handling and cooking index affected (P < 0.05) the probability of households testing positive for Listeria, regardless of presence of ruminants. For households with ruminants, personal cleanliness habits were related to presence of Listeria on shoes, clothes washing machine, and working gloves. Shoes testing positive in ruminant households were more frequently associated with multiple positive environmental samples, when compared to households without ruminants. Significance: Consumer education on handling and storing perishable foods and on animal handling to prevent contamination of the household through shoes or clothes may reduce prevalence of Listeria in home environments. Abstract Book — 187 Poster

IAFP 2009 Program Book and Abstract Book

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