IAFP 2009 Program Book and Abstract Book - AB25

T1-06 Enterobacteriaceae and Related Organisms Recovered from Biofilms in a Commercial Shell Egg Processing Facility MICHAEL T. MUSGROVE, Mark E. Berrang and Karen A. Liljebjelke USDA/ARS, Athens, GA, USA Introduction: Effective sanitation programs in shell egg sanitating facilities are essential to the production of wholesome eggs and decrease the risk of egg-borne disease. Purpose: Determine the identity of bacteria that contribute to biofilm formation in shell egg processing facilities and on egg and non-egg contact equipment surfaces. Methods: During six visits, biofilms from egg contact and non-contact surfaces in a commercial shell egg processing facility were sampled. Thirty-five different sample sites were selected: belts (farm, accumulator)pre-wash and wash tanks (lids, screens, tank interiors, nozzle guards, spindles), post-wash spindles, blower filters, scales, packer lanes (belts, brushes, sorters), plastic flat washers, and floor drains. Whirl-pak sponges moistened with 10 mL D/E neutralizing broth were used for aseptic to biofilm collection from each sample site. At the laboratory, aliquots were pour plated using violet red bile glucose agar (VRBG) with overlay and incubated at 37 C for 18 to 24 h. Enumerated results were described in a previous report. Isolates (1 to 3/plate) were selected randomly from plates exhibiting typical presumptive colonies. To increase the chance of isolate purity, each selected colony was restreaked three times on plate count agar incubated at 37°C for 18 to 24 h. An automated system using 95 biochemical tests was used to determine identifications to genus or species for 269 isolates. Results: Enterobacteriaceae genera recovered accounted for 91.4% (246/269) of identifications and included Buttiauxella, Cedecea, Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Leclercia, Morganella, Pantoea, Proteus, Providencia, Rahnella, Raoultella, Salmonella, Serratia, Shigella, and Xenorhabdus. Other genera recovered accounted for 8.6% (23/269) of the identifications and included: Aeromonas, Aquaspirillium, Pseudomonas, Vibrio, and Ralstonia. As has been reported previously, Enterobacter and Escherichia accounted for a majority of the identifications, 15.2 and 25.6%, respectively. Citrobacter, Klebsiella, and Raoultella accounted for 11.5, 12.6, and 11.2% of identifications. Aeromonas and Vibrio were associated with wash tank samples. Salmonella isolates were recovered from packer lane samples used to pack defective eggs (cracked shells, dirties, etc.) for shipment to egg breaking facilities. Significance: Understanding which genus or species contribute to biofilm contamination in commercial facilities can be used to develop more effective sanitation programs. T1-07 Identification of a Unique Food Safety Risk Associated with Retail Markets Serving Asian Populations in America DSC RENATA JACOB, Valerie L. Darcey, Trish A. Carney and Jennifer J. Quinlan Drexel University, Dept. of Biology, Philadelphia, PA, USA Introduction: Tofu is consumed by many and is especially popular with Asian populations. Previous research has examined its safety and found that if not handled properly, it may present a food safety risk. In this research, we examined the safety and prevalence of a practice in small Asian urban markets to sell fresh tofu from a “bucket” or open bin from which consumers who wish to purchase the product, remove the tofu cake and place it in a plastic bag. Purpose: This study examined the prevalence of this practice within the city of Philadelphia and the safety of the product sold using this practice as compared to traditionally pre-packaged tofu sold in the same markets. Methods: US Census Bureau data was used to identify tracts with high Asian populations in Philadelphia. Two databases were used to identify food store outlets in those tracts. Approximately 150 stores were identified and visited, and fifteen were found to practice the sale of tofu from “buckets” or open bins. These 15 stores were visited on multiple occasions and both “bucket” as well as prepackaged tofu samples obtained. Both tofu cakes and juice were tested for temperature, pH, Aerobic Plate Count, coliforms, fecal coliforms, E. coli, S. aureus and L. monocytogenes. Results: Results for 37 tofu samples and juice sold from the bucket were compared to 14 prepackaged samples and their juice. For tofu samples from buckets and packaged tofu, respectively: 73% and 28% had an aerobic plate count above 106 CFU/g; fecal coliforms were found in 19% and 7%; average temperature of storage was 49 and 45°F; presence of S. aureus was found in 3% of tofu from buckets; E. coli was found in 5% of juice from buckets. Significance: This study identified a common risky food handling practice for the sale of tofu in small Asian retail markets. T1-08 Sanitation Schedules – A New Management Approach JOHN T. HOLAH and Karen Middleton Campden BRI, Gloucestershire, United Kingdom Introduction: The continuing growth of the Ready-to-Eat (RTE) sector and the increasing desire to more effectively manage the sanitation processes to control pathogens, has led to a food industry review in the UK as to determine how best to document and manage sanitation, both on a routine basis and in response to a potential, environmental pathogen contamination incident. Purpose: A working party of food manufacturers, cleaning chemical suppliers, food retailers and Campden BRI met over the course of 18 months to optimise the management of the sanitation process, develop a number of sanitation schedules and propose guidance to food manufacturers on how the processes and schedules could be implemented. Methods: Sanitation schedules were developed that document the necessary work instructions that allow the successful completion of the sanitation task. The number of sanitation schedules required will depend on the size of the manufacturing plant, the risk or category of the food product and the complexity of the equipment to be cleaned. Whilst only 2 or 3 may be required for traditional food processors, up to 6 schedules can be adopted for RTE manufacturers, described as cleaning plan, wholeroom, in-production, end of production, periodic and site decontamination. Abstract Book — 25 Technical

IAFP 2009 Program Book and Abstract Book

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