IAFP 2009 Program Book and Abstract Book - AB46

Significance: This work is the first attempt to determine human illness food attribution from Canadian data. Multiple corres-pondence analyses was used to help clarify and visually highlight the food-pathogen associations contained in the causative agent and food vehicle cross-tabulation, while showing any change over time. T6-04 Enumeration of Campylobacter on Chickens at Processing and Retail: An Explanation of Regional Differences in Incidence of Campylobacter Observed in Foodnet Sites Mary Patrick, PRIYA KADAM, Reem Ghoneim, Hugh Maguire, Kirsten Larson, Trisha McDonald, Craig Braymen, Suzanne Solghan, Henrietta D. Hardin, Hannah Gould, Fred Angulo and James Rodgers Centers for Disease Control and Prevention, Enteric Diseases Epidemiology Branch, Atlanta, GA, USA Introduction: Over a million persons are infected with Campylobacter in the United States each year. Consumption of poultry is an important source of infection. The Foodborne Diseases Active Surveillance Network (FoodNet) measures the incidence of human Campylobacter infections in participating states. There are substantial variations in incidence between states, with higher incidence seen in the west. Purpose: To explore this variation, we compared the Campylobacter prevalence on chickens purchased at retail in the FoodNet sites to chickens collected at processing plants during the United States Dept. of Agriculture/Food Safety Inspection Service (FSIS) Young Chicken Baseline Study. Methods: During June to August 2008, fresh skin-on whole chickens were purchased at retail in nine FoodNet states. The plant code and sell-by date were recorded. The FSIS Young Chicken Baseline Study was conducted from June 2007 to July 2008. Chickens were rinsed in buffered peptone water and rinsates were enriched in Bolton broth and streaked onto Campy Cefex Agar. Campylobacter colonies were enumerated using direct plating onto Campy Cefex Agar. Results: For the FoodNet study, 270 chickens were purchased; 241 were analyzed. Of these, 143 (59%) yielded Campylobacter (range 1 to 310 colony forming units[CFU]/mL). The percentage of chickens which yielded Campylobacter varied by site from 75% in Tennessee and 68% in California to 44% in New York. In the FSIS Young Chicken Baseline Study, 65 (41%) of 159 chickens collected at processing plants from which the retail chickens tested in the FoodNet study originated yielded Campylobacter (range 1 to 3400 CFU/mL). Ninety percent of chickens from corresponding production plants in California yielded Campylobacter, compared with only 22% of chickens from plants in Connecticut. Significance: Regional differences in the incidence of Campylobacter infections may be partially explained by variations in Campylobacter prevalence on retail chickens. Further studies are needed to confirm these findings and to better understand contributing factors. T6-05 Reduction in Pathogen Reduction/Hazard Analysis Critical Control Point (PR/HACCP) Salmonella Positives at United States Food Safety and Inspection Service (FSIS)-regulated Broiler and Turkey Establishments PRIYA KADAM, Patty Bennett, Denise R. Eblen and Gurinder Saini USDA-FSIS, Washington, D.C., USA Introduction: The increase in broiler samples positive for Salmonella collected under PR/HACCP testing (from 11.5% in 2002 to 16.3% in 2005) prompted FSIS to introduce a risk-based Salmonella sample scheduling initiative in 2006. This initiative included the introduction of a risk-based algorithm to schedule Salmonella sampling, the categorization of establishments depending on the level of process control, as determined by Salmonella set results, and the introduction of PR/HACCP testing for turkey carcasses. Purpose: The numbers of Salmonella positives and the proportion of human health-hazard serotypes (hereafter “HHS”) as identified by CDC were compared for the first two years of risk-based sampling July 2006 to June 2007 (year 1-risk based:Y1RB) and July 2007 to June 2008 (year 2-risk based:Y2RB)) and the preceding year (non-risk based sampling: NRB) July 2005 to June 2006. Furthermore, the FSIS goal of having 90% of regulated establishments in category 1, i.e., demonstrating consistent process control by 2010, was evaluated. Methods: SAS software was used to extract, clean and analyze data from the FSIS Sybase data warehouse. Results: For NRB, Y1RB and Y2RB, 9369, 13,779 and 6,672 broiler samples and 407, 3806 and 423 turkey samples were collected, respectively. The percentage-positive Salmonella decreased from 13.4% in NRB to 8.2% in Y2RB for broilers, and from 10.1% in NRB to 7.1% in Y2RB for turkeys. On average, 4% of broiler samples had HHS Salmonella over the three years examined; for turkey, the percentage of such serotypes decreased from 8.1% in NRB to 4.7% in Y2RB. By the end of Y2RB, 83% of broiler establishments and 92% of turkey establishments were in category 1. Significance: These data suggest the introduction of this initiative had a positive impact on the reduction of Salmonella positive in broilers and turkeys. T6-06 Prevalence of Salmonella Species on Marinated Chicken Skin DSC AMRITA PATHANIA, Manpreet Singh and Shelly R. McKee Auburn University, Poultry Science, Auburn, AL, USA Introduction: Marination of meat products is widely done as it not only adds flavor for increased consumer acceptance but has been also reported to potentially enhance the safety and shelf life of meats. Poultry meat is known to harbor different spoilage and pathogenic bacteria such as, Salmonella, Campylobacter, Pseudomonas and Listeria spp. Purpose: A series of experiments were conducted to determine the effect of teriyaki and lemon pepper marinades on the persistence of multiple strains of nalidixic acid resistant Salmonella spp. Methods: Nalidixic acid resistant Salmonella (Typhimurium, Heidelberg, and Seftenberg) cultures were developed by inoculating BHI broth and incubating for 24 h at 37°C; subjecting strains to increasing concentrations of nalidixic acid in XLT4. As a result, (a) S. Typhimurium and S. Heidelberg resistant to 60 µgm of nalidixic acid, (b) S. Seftenberg resistant to Technical 46 — Abstract Book

IAFP 2009 Program Book and Abstract Book

Table of Contents for the Digital Edition of IAFP 2009 Program Book and Abstract Book