Liver & biliary 94.3%, sensitivity 100% (95% IC; 87.7% - 100%), specificity 89.5% (95% IC; 73.4% - 96.8%), PPV 88.9% (95% IC; 72.7% - 96.4%) and NPV 100% (95% IC; 87.7% - 100%). No adverse event were recorded using antibiotic prophylaxis for each case. Conclusion: DSOC represents a safe and valid tool for characterization of indeterminate biliary strictures combining the high sensitivity of visual finding with the specificity of direct biopsy sampling. Disclosure: S.M. Milluzzo: nothing to declare. A. Tringali: Boston Scientific. V. Perri: nothing to declare. P. Familiari: nothing to declare. I. Boskoski: Apollo Endosurgery, Cook Medical, Boston Scientific. R. Ricci: nothing to declare. G. Costamagna: Cook Medical, Olympus, Boston Scientific. 724 UEG Journal | Abstract Book